Package

r-sseq

Description

Shrinkage estimation of dispersion in Negative Binomial models for RNA-
seq experiments with small sample size. The purpose of this package is
to discover the genes that are differentially expressed between two
conditions in RNA-seq experiments. Gene expression is measured in counts
of transcripts and modeled with the Negative Binomial (NB) distribution
using a shrinkage approach for dispersion estimation. The method of
moment (MM) estimates for dispersion are shrunk towards an estimated
target, which minimizes the average squared difference between the
shrinkage estimates and the initial estimates. The exact per-gene
probability under the NB model is calculated, and used to test the
hypothesis that the expected expression of a gene in two conditions
identically follow a NB distribution.

Note